Determination of 4-vinylgaiacol and 4-vinylphenol in Top- fermented Wheat Beers by Isocratic High Performance Liquid Chromatography with Ultraviolet Detector
نویسندگان
چکیده
This work aimed at determining 4-vinylguaiacol and 4-vinylphenol in the top-fermented wheat beers using different wavelength and the mobile phase for HPLC. Best results for isocratic elution were obtained at 260 nm and the mobile phase comprising methanol/ultrapure water/phosphoric acid (400/590/10, V/V). Under these conditions, the retention time of 4-vinylguaiacol and 4-vinylphenol was 25 and 27min, respectively.
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